Flow cytometry
From BITS wiki
Contents
FlowJo introduction
Slides and tutorials:
- Schedule
- Introduction to flow cytometry
- FACS guidelines by Nature
- FlowJo10-2
- Compensation in flow cytometry
- text by Ryan Duggan on compensation
- text on compensation controls
- BD system for compensation
- UltraComp eBeads protocol
- FlowJo cell cycle tutorial
- FlowJo iteration & batching tutorial
- BD fluorochrome specs
- BD fluorochrome laser reference
- Overview of exercises
Files
Files for exercises in zip format.
Advanced FlowJo
FlowSOM
Material
- R notebook
- data in zip format
Software to install
The latest version of R and the latest version of RStudio and the follolwing
CRAN packages:
- devtools
- Rtsne
- tidyverse
- openxlsx
- mvtnorm
- BiocManager
Bioconductor packages:
- BiocManager::install()
- BiocManager::install("flowCore")
- BiocManager::install("flowWorkspace")
- BiocManager::install("flowAI")
- BiocManager::install("flowDensity")
- BiocManager::install("CytoML")
Github packages:
- library("devtools")
- devtools::install_github("saeyslab/FlowSOM")
- devtools::install_github("saeyslab/FlowSOM_workshop")
